Detection of Kelch13 and Coronin Genes in Colpodella sp. ATCC 50594

Detection of Kelch13 and Coronin Genes in Colpodella sp. ATCC 50594

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Colpodella species are predatory biflagellates phylogenetically related to pathogenic Apicomplexans.Following the attachment of Colpodella sp.to its prey, cytoplasmic contents of the prey are aspirated into a posterior food vacuole during myzocytosis.Trophozoites also endocytose nutrients as demonstrated by the uptake of 40 and 100 nm nanoparticles in Colpodella knob sp.ATCC 50594.

This nutrient uptake is actin-mediated.However, the markers of myzocytosis and endocytosis are unknown.Furthermore, the relationship between Colpodella sp.ATCC 50594 and Colpodella sp.identified in arthropods, humans, and animals are unknown.

In this study, we investigated the conservation of the coronin and Kelch 13 genes in Colpodella sp.ATCC 50594 using polymerase chain reaction (PCR).Kelch 13 distribution in Colpodella sp.ATCC 50594 was investigated using anti-Kelch 13 antibodies by immunofluorescence.Both genes were amplified from Colpodella sp.

ATCC 50594.We amplified DNA encoding 18S rRNA with similarity to 18S rRNA amplified using piroplasm primers from the Italian Colpodella sp.identified in cattle and ticks.The detection of the coronin and Kelch 13 genes in Colpodella sp.provides, for the first time, markers for actin binding and endocytosis in Colpodella species that can be investigated further to Coaster gain important insights into the mechanisms of nutrient uptake in Colpodella sp.